antibody

Anti-Actin

MBS500031

0.1 ml

235 USD

monoclonal

mouse

nonconjugated

C4

western blot, immunohistochemistry, immunocytochemistry

Antibody

Anti-Actin

Actin

actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; a-actin; alpha-actin-1; actin, alpha 1, skeletal muscle; Alpha-actin-1

ACTA1

ACTA1; ACTA1; ACTA

ACTS_CHICK

Monoclonal

IgG1

C4

Mouse

All

377

Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.

Ascites fluid (Unpurified ascites fluid.)

Liquid containing 0.01% sodium azide.

Store at -20 degree C in undiluted aliquots; stable for at lease one year. Avoid freeze/thaw cycles.

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB:1:5,000. IHC: 1:500. IF: 1:500

Antigen: Chicken gizzard actin. Immunogen Information: Preparation of chicken gizzard actin. Immunogen Species: Chicken

Species Reactivity Note: The antibody reacts with all known actins from yeast to man. Biological Significance: Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Mouse monoclonal antibody

Lessard, JL. Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motility and Cytoskeleton (1998) 10:349-362.

71894831

NP_001026234.1

NM_001031063.1

P68139

42

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Subcellular location: Cytoplasm cytoskeleton. Post-translational modification: Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization . By similarity. Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family.

0.1 ml

235 USD