antibody

Anti-Thyroid Hormone Receptor, beta-Isotype

MBS500022

0.1 mL

345 USD

monoclonal

mouse

nonconjugated

2386

Antibody

Anti-Thyroid Hormone Receptor, beta-Isotype

Thyroid Hormone Receptor, beta-Isotype

thyroid hormone receptor beta; Thyroid hormone receptor beta; thyroid hormone receptor beta; oncogene ERBA2; nuclear receptor subfamily 1 group A member 2; thyroid hormone nuclear receptor beta variant 1; thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); thyroid hormone receptor, beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta

THRB

THRB; THRB; GRTH; PRTH; THR1; ERBA2; NR1A2; THRB1; THRB2; C-ERBA-2; C-ERBA-BETA; ERBA2; NR1A2; THR1

THB_HUMAN

Monoclonal

IgG1

2386

Mouse

Human

461

Specific for the ~55k TR-beta protein.

Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, beta-isotype. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Human

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human tissue. Biological Significance: Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRalpha1, TRalpha2, TRbeta1 and TRbeta2. TRalpha1, TRbeta1 and TRbeta2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).

Affinity purified mouse monoclonal antibody

Ambrogini P, Cuppini R, Ferri P, Mancini C, Ciaroni S, Voci A, Gerdoni E, Gallo G (2005) Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology 81:244-253. Chan S, Kilby MD (2000) Thyroid hormone and central nervous system development. J Endocrinol 165:1-8. Constantinou C, Margarity M, Valcana T (2005) Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain. Mol Cell Biochem 278:93-100.

40806162

NP_000452.2

NM_000461.4

P10828

55

Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); SIDS Susceptibility Pathways (198901); Thyroid Hormone Signaling Pathway (946598)

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq, Jul 2008]

Function: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Ref.14 Ref.16 Ref.19 Ref.21 Ref.22 Ref.23 Ref.24. Subunit structure: Binds DNA as a dimer; homodimer and heterodimer with RXRA. Interacts with the coactivators NCOA1/SRC1, NCOA2/GRIP1, NCOA7 and MED1/TRAP220 in a ligand-inducible manner. Interacts with the corepressor NCOR1 in absence of ligand. Interacts with C1D . By similarity. Interacts with NR2F6; the interaction impairs the binding of the THRB homodimer and THRB:RXRB heterodimer to T3 response elements. Interacts with PRMT2 and THRSP. Ref.11 Ref.12 Ref.13 Ref.14 Ref.19 Ref.20 Ref.21 Ref.24. Subcellular location: Nucleus. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Involvement in disease: Generalized thyroid hormone resistance (GTHR) [MIM:188570]: A disease characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17 Ref.18 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.36 Ref.37 Ref.38 Ref.39 Ref.40 Ref.41 Ref.42 Ref.43Generalized thyroid hormone resistance autosomal recessive (GTHRAR) [MIM:274300]: An autosomal recessive disorder characterized by goiter, clinical euthyroidism, end-organ unresponsiveness to thyroid hormone, abnormal growth and bone maturation, and deafness. Patients also have high levels of circulating thyroid hormones, with elevated thyroid stimulating hormone.Note: The disease is caused by mutations affecting the gene represented in this entry.Selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]: Variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.35. Sequence similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain. Sequence caution: The sequence AAA35677.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAA28412.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

0.1 mL

345 USD