Anti-Rhodopsin | MyBioSource MBS500017 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Rhodopsin

catalog :

MBS500017

quantity :

0.1 ml

price :

270 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1D4

reactivity :

cow

application :

western blot, immunohistochemistry

product information

catalog number :

MBS500017

products type :

Antibody

products full name :

Anti-Rhodopsin

products short name :

Rhodopsin

other names :

rhodopsin; Rhodopsin; rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); rhodopsin<

products gene name :

RHO

other gene names :

RHO; RHO

uniprot entry name :

OPSD_BOVIN

clonality :

Monoclonal

isotype :

IgG1

clone :

1D4

host :

Mouse

reactivity :

Most mammalian

sequence length :

348

specificity :

Specific for the ~ 39k rhodopsin protein.

purity :

Protein G purified (Protein G purified culture supernatant.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

storage stability :

Store at -20 degree C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Quality Control: Western blots performed on each lot.Western blots performed on each lot. WB: 1:1000. IHC: 1:100

other info1 :

Antigen: Bovine rhodopsin. Immunogen Information: Purified native bovine rhodopsin. Immunogen Species: Bovine

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in mammals and amphibians. Biological Significance: Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.

products description :

Mouse monoclonal antibody

products references :

Berson EL, Rosner B, Sandberg MA, Dryja TP (1991). "Ocular findings in patients with autosomal dominant retinitis pigmentosa and a rhodopsin gene defect (Pro-23-His)". Arch. Ophthalmol. 109 (1): 92-101. Dryja TP, Berson EL, Rao VR, Oprian DD (1993) Heterozygous missense mutation in the rhodopsin gene as a cause of congenital stationary night blindness. Nature Genet. 4:280-283. Molday RS, MacKenzie D. (1983) Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes. Biochemistry. 22(3):653-60.

ncbi gi num :

62460472

ncbi acc num :

NP_001014890.1

ncbi gb acc num :

NM_001014890.1

uniprot acc num :

P02699

ncbi mol weight :

ncbi pathways :

Activation Of The Phototransduction Cascade Pathway 934280!!Class A/1 (Rhodopsin-like Receptors) Pathway 935232!!Disease Pathway 934178!!Diseases Associated With Visual Transduction Pathway 934272!!G Alpha (i) Signalling Events Pathway 935262!!GPCR Downstream Signaling Pathway 935260!!GPCR Ligand Binding Pathway 935231!!GPCRs, Class A Rhodopsin-like Pathway 920903!!Inactivation, Recovery And Regulation Of The Phototransduction Cascade Pathway 934281!!Integrin-mediated Cell Adhesion Pathway 198555

uniprot summary :

Function: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal . By similarity. Ref.26 Ref.27. Subunit structure: Homodimer. Ref.31. Subcellular location: Membrane; Multi-pass membrane protein. Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia . By similarity. Tissue specificity: Rod shaped photoreceptor cells which mediates vision in dim light. Post-translational modification: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Ref.10 Ref.18 Ref.19Contains one covalently linked retinal chromophore. Sequence similarities: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. Biophysicochemical propertiesAbsorption:Abs(max)=495 nm

size :

0.1 ml

price :

270 USD