antibody

Anti-Retinoid X Receptor, gamma-Isotype

MBS500016

0.1 ml

270 USD

monoclonal

mouse

nonconjugated

1373

human, rat

Antibody

Anti-Retinoid X Receptor, gamma-Isotype

Retinoid X Receptor, g-Isotype

retinoic acid receptor RXR-gamma isoform c; Retinoic acid receptor RXR-gamma; retinoic acid receptor RXR-gamma; nuclear receptor subfamily 2 group B member 3; retinoid X receptor, gamma; Nuclear receptor subfamily 2 group B member 3; Retinoid X receptor gamma

RXRG

RXRG; RXRG; RXRC; NR2B3; NR2B3

RXRG_HUMAN

Monoclonal

IgG1

1373

Mouse

Human, rat

340

Specific for the ~48k RXR-gamma isotype.

Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least 1 year

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Hinge peptide from human retinoid X receptor, gamma-isotype. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the hinge region conjugated to KLH. Immunogen Species: Human

Reactivity Assumed Based on 100% Sequence Homology: Bovine, mouse, non-human primate . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and human tissues. It is anticipated that the antibody will also work with bovine, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Retinoic acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The Retinoid X Receptors (RXRs) family (RXRalpha, beta and gamma) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).

Affinity purified mouse monoclonal antibody

Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720. Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027. Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293.

375151581

NP_001243500.1

NM_001256571.1

P48443

48

Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Non-small Cell Lung Cancer Pathway 83119!!Non-small Cell Lung Cancer Pathway 531!!Nuclear Receptor Transcription Pathway 105979!!Nuclear Receptors Pathway 198848!!PPAR Signaling Pathway 83042

This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the antiproliferative effects of retinoic acid (RA). This receptor forms dimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene is expressed at significantly lower levels in non-small cell lung cancer cells. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]

Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid . By similarity. Subunit structure: Homodimer . By similarity. Heterodimer with a RAR molecule . By similarity. Binds DNA preferentially as a RAR/RXR heterodimer . By similarity. Subcellular location: Nucleus . By similarity. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain . By similarity. Sequence similarities: Belongs to the nuclear hormone receptor family. NR2 subfamily.Contains 1 nuclear receptor DNA-binding domain.

0.1 ml

270 USD