antibody

Anti-Retinoic Acid Receptor, beta-Isotype

MBS500014

0.1 ml

270 USD

monoclonal

mouse

nonconjugated

336

human, rat

Antibody

Anti-Retinoic Acid Receptor, beta-Isotype

Retinoic Acid Receptor, beta-Isotype

retinoic acid receptor beta isoform 1; Retinoic acid receptor beta; retinoic acid receptor beta; RAR-beta; RAR-epsilon; HBV-activated protein; retinoic acid receptor beta 2; retinoic acid receptor beta 4; retinoic acid receptor beta 5; hepatitis B virus activated protein; retinoic acid receptor beta variant 1; retinoic acid receptor beta variant 2; retinoic acid receptor, beta polypeptide; nuclear receptor subfamily 1 group B member 2; retinoic acid receptor, beta; HBV-activated protein; Nuclear receptor subfamily 1 group B member 2; RAR-epsilon

RARB

RARB; RARB; HAP; RRB2; NR1B2; MCOPS12; HAP; NR1B2; RAR-beta

RARB_HUMAN

Monoclonal

IgG1

336

Mouse

Human, rat

448

Specific for the ~48k RAR-beta isotype.

Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

Store at -20 degree C; stable for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Peptide corresponding to amino acids from N-terminal region of human retinoic acid receptor, beta-isotype. The antigen is identical in both beta1 and beta2 isoforms. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Human

Reactivity Assumed Based on 100% Sequence Homology: Canine, guinea pig, mouse and non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in human and rat tissues. It is anticipated that the antibody will also work with canine, guinea pig, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells (Joshi et al., 2005). The Retinoid X receptors (RXRs) family (RXRalpha, beta and gamma) preferentially bind 9-cis-RA and regulate gene transcription by forming heterodimers with a second family of RA receptors (RARs). RAs have been suggested to potentially play a therapeutic role in cervical cancer (Abu et al., 2005). RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors (Lane and Bailey, 2005).

Affinity purified mouse monoclonal antibody

Abu J, Batuwangala M, Herbert K, Symonds P (2005) Retinoic acid and retinoid receptors: potential chemopreventive and therapeutic role in cervical cancer. Lancet Oncol 6:712-720. Joshi S, Guleria R, Pan J, Dipette D, Singh US (2005) Retinoic acid receptors and tissue-trans-glutaminase mediate short-term effect of retinoic acid on migration and invasion of neuroblastoma SH-SY5Y cells. Oncogene advance online publication 12 September 2005; doi: 10.1038/sj.onc.1209027. Lane MA, Bailey SJ (2005) Role of retinoid signalling in the adult brain. Prog Neurobiol 75:275-293.

14916494

NP_000956.2

NM_000965.3

P10826

48

Gene Expression Pathway 105937!!Generic Transcription Pathway 105938!!Integrated Pancreatic Cancer Pathway 711360!!Non-small Cell Lung Cancer Pathway 83119!!Non-small Cell Lung Cancer Pathway 531!!Nuclear Receptor Transcription Pathway 105979!!Nuclear Receptors Pathway 198848!!Nuclear Receptors In Lipid Metabolism And Toxicity Pathway 198887!!Pathways In Cancer 83105!!Small Cell Lung Cancer Pathway 83118

This gene encodes retinoic acid receptor beta, a member of the thyroid-steroid hormone receptor superfamily of nuclear transcriptional regulators. This receptor localizes to the cytoplasm and to subnuclear compartments. It binds retinoic acid, the biologically active form of vitamin A which mediates cellular signalling in embryonic morphogenesis, cell growth and differentiation. It is thought that this protein limits growth of many cell types by regulating gene expression. The gene was first identified in a hepatocellular carcinoma where it flanks a hepatitis B virus integration site. The gene expresses at least two transcript variants; one additional transcript has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]

Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Ref.10. Subunit structure: Homodimer . By similarity. Heterodimer; with a RXR molecule. Binds DNA preferentially as a RAR/RXR heterodimer . By similarity. Interacts weakly with NCOR2. Ref.10. Subcellular location: Isoform Beta-1: Nucleus. Isoform Beta-2: Nucleus. Isoform Beta-4: Cytoplasm. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Sequence similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain. Sequence caution: The sequence CAA27637.1 differs from that shown. Reason: Erroneous gene model prediction.

0.1 ml

270 USD