Anti-Neurofilament L (NF-L) | MyBioSource MBS500007 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Neurofilament L (NF-L)

catalog :

MBS500007

quantity :

0.1 mL

price :

300 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DA2

reactivity :

human, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS500007

products type :

Antibody

products full name :

Anti-Neurofilament L (NF-L)

products short name :

Neurofilament NF-L ms

other names :

Neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein

products gene name :

NEFL

other gene names :

NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; NF-L

uniprot entry name :

NFL_PIG

clonality :

Monoclonal

isotype :

IgG1

clone :

DA2

host :

Mouse

reactivity :

Rat

sequence length :

549

specificity :

Specific for the ~68k Neurofilament L protein.

purity :

Total IgG fraction (Unpurified, concentrated culture supernatant.)

form :

Liquid containing 10mM sodium azide

storage stability :

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1,000. IF: 1:100

other info1 :

Antigen: Preparation of porcine intermediate filaments. Immunogen Information: Preparation of porcine NF-L. Immunogen Species: Pig

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Chicken, human, mouse. Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues. Biological Significance: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

products description :

Mouse monoclonal antibody

products references :

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.

ncbi gi num :

143811426

ncbi acc num :

P02547.3

uniprot acc num :

P02547

ncbi mol weight :

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Subunit structure: Interacts with ARHGEF28 . By similarity. Interacts with TRIM2 . By similarity. Domain: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. Post-translational modification: O-glycosylated . By similarity.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization . By similarity.Ubiquitinated in the presence of TRIM2 and UBE2D1 . By similarity. Miscellaneous: NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Sequence similarities: Belongs to the intermediate filament family.

size1 :

0.1 mL

price1 :

300 USD

more info or order :

MyBioSource product webpage