antibody

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

MBS500004

0.1 ml

235 USD

monoclonal

mouse

nonconjugated

1D4

human, mouse, rat

western blot, immunocytochemistry

Antibody

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Glyceraldehyde 3-Phosphate Dehydrogenase

glyceraldehyde-3-phosphate dehydrogenase, testis-specific; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; spermatogenic glyceraldehyde-3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; GAPDH-2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

GAPDHS

GAPDHS; GAPDHS; GAPD2; GAPDS; HSD-35; GAPDH-2; GAPD2; GAPDH2; GAPDS; GAPDH-2

G3PT_HUMAN

Monoclonal

IgM

1D4

Mouse

Human, mouse, rat

408

Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Total IgM fraction--concentrated culture supernatant (Unpurified, concentrated culture supernatant.)

Liquid. Contains 10 mM sodium azide.

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Western Blot (WB), Immunofluorescence (IF)

Quality Control: Western blots performed on each lot. WB: 1:1,000. IF: 1:100

Antigen: Extensively purified pig GAPDH. Immunogen Information: Purified porcine GAPDH. Immunogen Species: Porcine

Reactivity Assumed Based on 100% Sequence Homology: Avian, bovine. Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, chicken, human, mouse, porcine and rat tissues. Biological Significance: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4).

Mouse monoclonal antibody

Morgenegg G, Winkler GC, Hubscher U, Heizmann CW, Mous J, Kuenzle CC. Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. J Neurochem. 47:54-62 (1986). Schulze H, Schuler A, Stuber D, Dobeli H, Langern H & Huber G. Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's beta-amyloid precursor protein. J Neurochem. 60:1915-22 (1993). Burke JR, Enghild JJ, Martin ME, Jou Y-S, Myers RM, Roses AD, Vance JM & Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Med. 2: 347-350 (1996). Dastoor Z. & Dreyer, J-L. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. J. Cell Sci. 114:1643-1653 (2001).

7657116

NP_055179.1

NM_014364.4

O14556

38

Gluconeogenesis Pathway 106204!!Glucose Metabolism Pathway 106199!!Glycolysis Pathway 105911!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Glycolysis And Gluconeogenesis Pathway 198814!!Metabolism Pathway 477135!!Metabolism Of Carbohydrates Pathway 106196!!Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway 835393!!Gluconeogenesis Pathway 782382

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]

Function: May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility . By similarity. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Ref.7 Ref.8 Ref.9. Subcellular location: Cytoplasm . By similarity. Tissue specificity: Testis specific. Domain: The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components (Ref.8). Ref.8. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

0.1 ml

235 USD