ELISA/assay

Cetuximab Antibody ELISA Kit

MBS495348

96 Tests

1025 USD

ELISA Kit

Cetuximab Antibody ELISA Kit

[Cetuximab]

Based on the approach used in calculating the assay cut-off value of anti-drug antibody (ADA) ELISAs, approximately 1-5% of tested samples are expected to generate false-positive ADA response during initial screening analysis. Therefore, further confirmation of the specificity of the ADA activity in the samples, identified as positive, is highly recommended. This is supported also by recent publications. In order to confirm the specificity of the ADA signal, a confirmatory assay (competitive drug inhibition test) is proposed by ImmunoGuide. The confirmatory assay is performed with the Confirmatory Reagent (coded IG-CR112). This Confirmatory Reagent is available as a separate product and can be obtained from ImmunoGuide upon request.

2 - 8 degree C

Samples: Serum, Urine And Platelets. Assay Type: Quantitative Competitive

Immunology; Research Assays; Assay Categories

Intended Uses: Enzyme Immunoassay for the quantitative determination of Serotonin in serum, urine and platelets. Principle of the Assay: In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

96 Tests

1025 USD