ELISA/assay

West Nile Virus IgG ELISA Kit

MBS495333

96 Tests

365 USD

ELISA Kit

West Nile Virus IgG ELISA Kit

[West Nile Virus IgG]

[IgG]

Human

A total of 825 stool specimens were tested in parallel with the vero-cell cytotoxicity assay and the Verotoxin 1+2. The sample material consisted of 795 specimens sent for TPE-group analysis and 30 samples that were already characterized by shiga-toxin gen PCR and culture and stored at-20 degree C until testing. Enrichment culture (mTSB; 18-20 hours at 37 degree C) of all samples was carried out prior to ELISA testing.

2 - 8 degree C

Samples: Stool Specimens

Infectious Disease; Research Assays; Assay Categories

Intended Uses: The Verotoxin 1 + 2 ELISA is an enzyme immunoassay for the direct detection of Verotoxin 1 and 2 (shiga-toxin 1 and 2) in faecal samples and stool culture supernatants. For Research Use Only. Not for Use in Diagnostic Procedures. Principle of the Assay: Verotoxin 1+2 is an indirect two-site-immunoassay for the qualitative determination of verotoxin 1 and 2 based on polyclonal and monoclonal antibodies. Verotoxin 1 and/or 2 of specimens and the positive control react with polyclonal anti-verotoxin 1 and 2 antibodies coated on the solid phase of the microplate. After incubation for 60 minutes at room temperature (RT) non-bound material is removed by a washing step. Subsequently bound toxins specifically react with biotinylated monoclonal anti-verotoxin 1 and anti-verotoxin 2 antibodies during a second incubation period of 30 min at RT. Non-bound material is separated from the solid-phase immune complexes by a subsequent washing step. During the next incubation period of 30 min at RT horseradish peroxidase (HRP) conjugated streptavidin reacts with the bound biotinylated antibodies. Unbound conjugate is removed by a washing step. HRP converts the subsequently added colourless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells after 15 min incubation at RT turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of verotoxin 1 and/or verotoxin 2. Considering the cut-off value results are interpreted as positive or negative!!Background/Introduction: Invasive and toxigenic Escherichia coli strains cause diarrhoea in infants and adults. Among pathogenic E. coli strains the group of enterohaemorrhagic E. coli (EHEC) can cause lifethreatening haemorrhagic colitis and haemolytic uraemic syndrome (HUS) leading to acute renal failure and haemolytic anaemia with thrombocytopenia (1, 2, 3). Strains like E. coli O:157; O:26; O:111 and other serovars are characterized by the production of cytotoxins (verotoxin 1 and 2 or shiga-toxin 1 and 2, shiga-toxin variants). Immunological methods like enzyme immunoassay enable a fast and specific shiga-toxin detection in stool samples. It is commonly recommended to enrich the EHEC bacteria in selective broth media prior to the test run to enhance the sensitivity of the method (4, 5, 6).

96 Tests

365 USD