ELISA/assay

Kynurenine ELISA

MBS495082

96 Tests

700 USD

ELISA Kit

Kynurenine ELISA

[Kynurenine]

Human

2 - 8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Whole Blood. Assay Type: Competitive

Immunology; Cytokines & Cell Signalling; Oncology; Research Assays; Assay Categories

Intended Uses: Enzyme Immunoassay for the determination of Histamine in urine and cell culture samples and for the determination of Total Histamine in whole blood. For research use only, not for use in diagnostic procedures. First, Histamine is acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations. Principle of the Assay: Enzyme Immunoassay for the determination of Histamine in urine and cell culture samples and for the determination of Total Histamine in whole blood. For research use only, not for use in diagnostic procedures. First, Histamine is acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations!!Background/Introduction: Histamine belongs to the biogenic amines and is synthesized by decarboxylation from the amino acid histidine. It is synthesized by mast cells, basophils, platelets, histaminergic neurons, and enterochromaffine cells, where it is stored intracellularly in vesicles and released on stimulation. Histamine acts by binding to its 4 receptors (H1R, H2R, H3R and H4R) on target cells in various tissues. It causes smooth muscle cell contraction, vasodilatation, increased vascular permeability and mucus secretion, tachycardia, alterations of blood pressure, and arrhythmias. In humans, histamine is one of the most important mediators and takes part in the initial phase of an anaphylactic reaction ("immediate type" allergy). Of interest is also the determination of the histamine release from basophilic leucocytes in allergies.

96 Tests

700 USD