catalog number :
MBS495060
products type :
ELISA Kit
products full name :
Neopterin ELISA
products short name :
[Neopterin]
storage stability :
2 - 8 degree C
other info1 :
Samples: Urine. Assay Type: Competitive
products categories :
Immunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
products description :
Intended Uses: Enzyme Immunoassay for the determination of Metanephrine and Normetanephrine in urine. For research use only, not for use in diagnostic procedures. Principle of the Assay: During the sample preparation Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations!!Background/Introduction: Metanephrine and Normetanephrine are the metabolites of the catecholamines Epinephrine and Norepinephrine, respectively. They are metabolized to Vanillylmandelic acid or excreted with the urine. Subjects with pheochromocytoma or other tumors derived from neuroendocrine cells show elevated urinary levels of total Metanephrines. As catecholamine secretion from neuroendocrine cells might show high variations, urine samples collected over a period of 24 hours are used to average these fluctuations.