ELISA/assay

Granzyme B (Human) ELISA

MBS494561

96 Tests

680 USD

ELISA Kit

Granzyme B (Human) ELISA

Granzyme B

granzyme B; Granzyme B; granzyme B; C11; CTLA-1; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic serine protease B; fragmentin 2; fragmentin-2; human lymphocyte protein; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

GZMB; GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

GRAB_HUMAN

Human

247

2 - 8 degree C

Samples: Cell Culture Supernatant and serum. Detection Range: 0.7 - 480.0 pg/mL

Standards: 7 calibrators, serially diluted from 1 prepared lyophilized calibrator. Sample Volume: 50 uL / determination. Design: Enzyme-linked immunosorbent assay (ELISA). Assay Description: 1 hour incubation (RT) + 1 hour (RT) + 30 min. (RT) + 15 min. (RT) = 2 hours, 45 min. total incubation time.

Cytokines & Cell Signalling; Research Assays; Assay Categories

Enzyme-linked immunosorbent assay (ELISA) for the determination of human Granzyme B in cell culture supernatant and serum. For research use only, not for use in diagnostic procedures.

181186

AAA75490.1

P10144

27,716 Da

Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway (105657); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018)

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: EC 3.4.21.79; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; immunological synapse; nucleus; cytosol. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; apoptosis; natural killer cell mediated cytotoxicity; cytolysis; protein processing; immune response; proteolysis

96 Tests

680 USD