ELISA/assay

Measles IgG ELISA

MBS494527

96 Tests

275 USD

ELISA Kit

Measles IgG ELISA

Measles IgG

Human

2 - 8 degree C

Samples: Serum and plasma (EDTA, heparin). Detection Range: 4 standards expressed in AU/ can be used qualitatively with cutoff standard. Sensitivity: 1.35 U/mL

Standards: 4 standards diluted with PBS, ready to use. Sample Volume: 5 uL. Design: Enzyme Immunoassay technique. Controls: Optional use of standards as controls if cutoff standard is used. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Diagnostic Assays; Assay Categories

Intended Use The Measles IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against Measles in serum and plasma. This assay can be used for in-vitro diagnostic procedures. PRINCIPLE OF THE TEST The Measles IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Measles antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Measles antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman- IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

96 Tests

275 USD