IL-10 (Human) ELISA | MyBioSource MBS494524 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

IL-10 (Human) ELISA

catalog :

MBS494524

quantity :

96 Tests

price :

680 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS494524

products type :

ELISA Kit

products full name :

IL-10 (Human) ELISA

products short name :

IL-10

other names :

interleukin-10; Interleukin-10; interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor; interleukin 10; Cytokine synthesis inhibitory factor; CSIF

products gene name :

IL-10

other gene names :

IL10; IL10; CSIF; TGIF; GVHDS; IL-10; IL10A; IL-10; CSIF

uniprot entry name :

IL10_HUMAN

sequence length :

178

products description :

Intended Use The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the detection of human IL-10. For research use only, not for use in diagnostic procedures. PRINCIPLE OF THE TEST An anti-human IL-10 coating antibody is adsorbed onto microwells. Human IL-10 present in the unknown or calibrator binds to antibodies adsorbed to the microwells. A biotin-conjugated anti-human IL-10 antibody is added and binds to human IL-10 captured by the first antibody. Following incubation unbound biotin-conjugated anti-human IL-10 antibody is removed during a wash step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human IL-10 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human IL-10 present in the unknown or calibrator. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A calibrator curve is prepared from 7 human IL-10 calibrator dilutions and human IL-10 unknown concentration determined.

ncbi gi num :

10835141

ncbi acc num :

NP_000563.1

ncbi gb acc num :

NM_000572.2

uniprot acc num :

P22301

ncbi mol weight :

20,517 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121)

ncbi summary :

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]

uniprot summary :

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family. Protein type: Cytokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding. Biological Process: B cell proliferation; regulation of isotype switching; negative regulation of chronic inflammatory response to antigenic stimulus; leukocyte chemotaxis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; cytoplasmic sequestering of NF-kappaB; negative regulation of T cell proliferation; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; regulation of sensory perception of pain; response to activity; receptor biosynthetic process; negative regulation of apoptosis; positive regulation of cytokine secretion. Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To

size1 :

96 Tests

price1 :

680 USD

more info or order :

MyBioSource product webpage