ELISA/assay

PSA (Total) ELISA

MBS494521

96 Tests

255 USD

ELISA Kit

PSA (Total) ELISA

PSA

prostate-specific antigen isoform 1 preproprotein; Prostate-specific antigen; prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin; kallikrein-related peptidase 3; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase

PSA

KLK3; KLK3; APS; PSA; hK3; KLK2A1; APS; PSA; Seminin

KLK3_HUMAN

Human

261

2 - 8 degree C

Samples: Serum and plasma

Oncology; Research Assays; Assay Categories

Intended Uses The Measles IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Measles in serum and plasma. For in-vitro diagnostic use. Principle of the Assay: The Measles IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Measles antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Measles antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-lgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the evelopment of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

4502173

NP_001639.1

NM_001648.2

P07288

25,116 Da

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Metabolism Of Proteins Pathway (106230); Pathways In Cancer (83105); Prostate Cancer Pathway (755440); Prostate Cancer Pathway (83111); Prostate Cancer Pathway (523); Regulation Of Androgen Receptor Activity Pathway (138027); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily. Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.77. Chromosomal Location of Human Ortholog: 19q13.41. Cellular Component: extracellular region; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis

96 Tests

255 USD