ELISA/assay

Brucella-IgG ELISA

MBS494506

96 Tests

275 USD

ELISA Kit

Brucella-IgG ELISA

Brucella-IgG

Human

2 - 8 degree C

Standard Range: 4 standards expressed in AU/ can be used qualitatively with cutoff standard. Standards: 4 standards diluted with PBS, ready to use. Sensitivity: 1.11 U/mL. Sample Volume: 5 uL. Sample Types: Serum, plasma (EDTA, heparin)

Design: Enzyme Immunoassay technique. Controls: Optional use of standards as controls if cutoff standard is used. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Diagnostic Assays; Assay Categories

Intended Use The Brucella IgG Antibody ELISA Test Kit has been designed for the the detection of IgG class antibodies against Brucella in serum and plasma. For in-vitro diagnostic use. Principles of the Test The Brucella IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Brucella antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Brucella antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman- IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

96 Tests

275 USD