ELISA/assay

CRP (Mouse) ELISA

MBS494481

96 Tests

525 USD

ELISA Kit

CRP (Mouse) ELISA

CRP

CRP; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

CRP

CRP; CRP; PTX1; PTX1

CRP_HUMAN

Mouse

224

2 - 8 degree C

Standard Range: 0 / 1.56 - 25 ng/mL. Standards: 5 standards, serially diluted. Sensitivity: 2.5 ng/mL. Sample Volume: Serum: 10 uL. Sample Types: Mouse serum, plasma, urine, culture supernatant, tissue extracts, synovial fluid.

Design: Enzyme immunoassay technique. Assay Description: 1 hour (RT) + 30 min. (RT) + 10 min. (RT) = 1 hour, 40 min. total clock-time

Cytokines & Cell Signalling; Research Assays; Assay Categories

Intended Use The C-reactive protein assay is intended for the detection of mouse C-reactive protein (CRP) in mouse serum, plasma, and fluids. Principle of the Test Mouse sera/plasma for testing are diluted to 1:20 (suggested) and allowed to react with pneumococcal Cpolysaccharide coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzyme-labeled rabbit anti-CRP (conjugate) is then added to react with and tag the antigen-antibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450 nm on a spectrophotometer or ELISA reader. Semiquantification of absorbance can be accomplished by the use of a standard curve generated by measuring two-fold dilutions of the standard provided.

17975775

AAL48218.2

P02741

10,415 Da

Classical Antibody-mediated Complement Activation Pathway 106409!!Complement Cascade Pathway 106405!!Creation Of C4 And C2 Activators Pathway 106407!!IL6-mediated Signaling Events Pathway 137932!!Immune System Pathway 106386!!Initial Triggering Of Complement Pathway 106406!!Innate Immune System Pathway 106387!!Selenium Pathway 198825

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

96 Tests

525 USD