ELISA/assay

Varicella zoster IgG ELISA

MBS494474

96 Tests

260 USD

ELISA Kit

Varicella zoster IgG ELISA

Varicella zoster IgG

Human

2 - 8 degree C

Samples: Serum and plasma

Infectious Disease; Immunology; Research Assays; Assay Categories

Intended Uses: The Varizella zoster IgG Antibody ELISA Test Kit has been designed for the determination of specific IgG antibodies against Varizella zoster in serum and plasma. For research use only, not for use in diagnostic procedures. Principle of the Assay: The Varizella zoster IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Varizella zoster antigen is bound on the surface of the microtiter strips. Diluted serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Varizella zoster antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-lgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

96 Tests

260 USD