ELISA/assay

Influenza A IgG ELISA

MBS494380

96 Tests

260 USD

ELISA Kit

Influenza A IgG ELISA

Influenza A IgG

Human

2 - 8 degree C

Samples: Serum and plasma. Detection Range: 1 - 150 U/mL. Sensitivity: 1.09 U/mL; Clinical sensitivity: 100%

Standards: 4 calibrators, ready to use. Sample Volume: 100 uL of the diluted (1:101) sample / determination. Design: Enzyme Immunoassay technique. Assay Description: 60 min. incubation (RT) + 30 min. (RT) + 20 min. (RT) = 1 hour, 50 min. total incubation time.

Infectious Disease; Immunology; Diagnostic Assays; Assay Categories

Intended Use The Influenza A IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against Influenza A in serum and plasma. For in vitro diagnostic use only. PRINCIPLE OF THE TEST The MBS Influenza A IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman- IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color rom blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.

96 Tests

260 USD