ELISA/assay

Candida albicans IgA ELISA

MBS494353

96 Tests

275 USD

ELISA Kit

Candida albicans IgA ELISA

albicans IgA

Human

2 - 8 degree C

Sample Volume: 5 uL. Sample Types: Serum, plasma (EDTA, heparin)

Design: Enzyme Immunoassay technique. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Research Assays; Assay Categories

Intended Use The Candida albicans IgA Antibody ELISA Test Kit has been designed for the determination of specific IgA antibodies against Candida albicans in serum and plasma. For research use only, not for use in diagnostic procedures. Principles of the Test The MBS Candida albicans IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Candida albicans antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Candida albicans antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then readyto- use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.

96 Tests

275 USD