ELISA/assay

Mycobacterium Tuberculosis IgM ELISA

MBS494348

96 Tests

275 USD

ELISA Kit

Mycobacterium Tuberculosis IgM ELISA

Mycobacterium Tuberculosis IgM

Human

2 - 8 degree C

Sample Volume: 5 uL. Sample Types: Serum and plasma (EDTA, heparin)

Design: Enzyme Immunoassay technique. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Research Assays; Assay Categories

Intended Use The Mycobacterium tuberculosis IgM antibody ELISA kit has been designed for the detection of IgM class antibodies against Mycobacterium tuberculosis in serum and plasma. For research use only, not for use in diagnostic procedures. Principles of the Test The Mycobacterium tuberculosis IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Mycobacterium tuberculosis antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. NOTE that unknowns containing abnormally elevated levels of anti-human IgG antibodies may cause inaccurate results in this assay. Any unknowns showing elevated results should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM.

96 Tests

275 USD