ELISA/assay

Epstein-Barr (VCA) IgG ELISA

MBS494306

96 Tests

275 USD

ELISA Kit

Epstein-Barr (VCA) IgG ELISA

Epstein-Barr IgG

Human

2 - 8 degree C

Samples: Serum and plasma

Design: Enzyme Immunoassay technique. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Research Assays; Assay Categories

Intended Use The MBS EBV VCA IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against EBV VCA in serum and plasma. For research use only, not for use in diagnostic procedures. . Principle of the Test The MBS EBV VCA IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). EBV VCA antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized EBV VCA antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.

96 Tests

275 USD