ELISA/assay

HSV 1 IgG ELISA

MBS494292

96 Tests

275 USD

ELISA Kit

HSV 1 IgG ELISA

HSV 1 IgG

HSV 1

Human

2 - 8 degree C

Sample Volume: 5 uL. Sample Types: Serum and plasma (EDTA, heparin)

Design: Enzyme Immunoassay technique. Assay Description: 1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)

Infectious Disease; Immunology; Research Assays; Assay Categories

Intended Use The Herpes 1 IgG Antibody ELISA Test Kit has been designed for the detection of IgG class antibodies against Herpes 1 in serum and plasma. For research use only, not for use in diagnostic procedures. Principle of the Test The Herpes 1 IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Herpes 1 antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Herpes 1 antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.

96 Tests

275 USD