Osteocalcin ELISA | MyBioSource MBS494060 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Osteocalcin ELISA

catalog :

MBS494060

quantity :

96 Tests

price :

800 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS494060

products type :

ELISA Kit

products full name :

Osteocalcin ELISA

products short name :

Osteocalcin

other names :

osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

other gene names :

BGLAP; BGLAP; OC; BGP; OCN; BGP

uniprot entry name :

OSTCN_HUMAN

reactivity :

Human

sequence length :

100

specificity :

This method detects intact human osteocalcin. N-terminal and C-terminal fragments have been tested at their maximum levels found in normal and pathological samples, were added to a low and a high value calibrator. No cross reactivity was observed at these concentrations.

storage stability :

2 - 8 degree C

other info1 :

Samples: Serum. Assay Type: Sandwich

products categories :

Endocrinology ; Hormones; Research Assays; Assay Categories

products description :

Intended Uses: Immunoenzymetric assay for the determination of intact human Osteocalcin in serum. For Research Use Only - Not for Use in Diagnostic Procedures. Principle of the Assay The Osteocalcin human ELISA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on breakable microtiterplates. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of human osteocalcin. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human osteocalcin - MAb 2 - HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzyme-labelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the osteocalcin concentration. A calibration curve is plotted and osteocalcin concentration in samples is determined by interpolation from the calibration curve.

ncbi gi num :

40316933

ncbi acc num :

NP_954642.1

ncbi gb acc num :

NM_199173.4

uniprot acc num :

P02818

ncbi mol weight :

10,963 Da

ncbi pathways :

FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Retinoblastoma Protein Pathway (137916); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

ncbi summary :

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

uniprot summary :

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted, signal peptide; Cell development/differentiation; Secreted. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to vitamin D; response to ethanol; regulation of osteoclast differentiation; response to vitamin K; response to mechanical stimulus; response to zinc ion; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

size1 :

96 Tests

price1 :

800 USD

more info or order :

MyBioSource product webpage