ELISA/assay

IL-6 (Rat) ELISA Kit

MBS490363

96 Tests

710 USD

ELISA Kit

IL-6 (Rat) ELISA Kit

[IL-6]

[interleukin-6; Interleukin-6; interleukin-6; interleukin 6]

[IL-6]

[Il6; Il6; ILg6; Ifnb2; Il-6; IL-6]

IL6_RAT

Rat

211

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Body Fluids, Tissue Homogenate Or Cell Culture Supernates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: <5 pg/ml

Intended Uses: The rat IL-6 ELISA has been designed for the precise analytical measurement of IL-6 concentrations in serum, body fluids, tissue homogenate or cell culture supernates from rat. For research use only, not for use in diagnostic procedures. Principle of the Assay: This kit is based on a sandwich enzyme-linked immune-sorbent assay technology. An analyte-specific polyclonal antibody is pre-coated onto 96-well plates. The biotin conjugated second antibody is used as detection antibody. During the first incubation the standards and samples react with the analyte-specific pre-coated antibody. In a second incubation the biotin conjugated detection antibody completes the sandwich. After washing with wash buffer the TMB substrate is added to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The optical density of the yellow color is proportional to the analyte captured in plate. Read the O.D. absorbance at 450nm in a microtiterplate reader, and calculate the concentration of the analyte in the sample by taking into consideration the dilution factor of the sample!!Background/Introduction: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body's temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer's Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.

7549769

NP_036721.1

NM_012589.2

P20607

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (829152); Cytokine-cytokine Receptor Interaction Pathway (83443)

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

96 Tests

710 USD