antibody

Anti-Human Muscle Acetylcholine Receptor [mAb 192]

MBS488314

0.2 mg

425 USD

monoclonal

rat

nonconjugated

[mAb 192]

human, mouse, rat

western blot, ELISA, neutralization, enzyme immunoassay

Antibody

Anti-Human Muscle Acetylcholine Receptor [mAb 192]

[Muscle Acetylcholine Receptor]

[AChR]

[acetylcholine receptor subunit alpha isoform b; Acetylcholine receptor subunit alpha; acetylcholine receptor subunit alpha; cholinergic receptor nicotinic alpha 1 subunit]

[AChR]

[CHRNA1; CHRNA1; ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS; ACHRA; CHNRA]

Monoclonal

IgG2b, Kappa

[mAb 192]

Rat

Human, Mouse, Rat

457

The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM.

Protein A Affinity Purified

PBS with 0.02% Proclin 300.

1 mg/ml

Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at-20 degree C.

ELISA (EIA), Western Blot (WB), Blocking (BL)

This antibody binds to the human muscle Acetylcholine receptor, which is a ligand-gated ion channel, transducing an action potential into the muscle cells. The antibody binds to the main immunogenic region (MIR) and prevents ligand-binding.

Immunogen: Human muscle AChR.

Physiology; Electrophysiology; Neuromuscular Disorders

Recombinant monoclonal antibody to Human Muscle Acetylcholine Receptor. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAb 192. Human muscle AChR.

Kontou et al. Characterisation, crystallisation and preliminary X-ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor. FEBS Letters 1996; 389(2):195-198 PMID:8766828

4557457

NP_000070.1

NM_000079.3

P02708

Acetylcholine Binding And Downstream Events Pathway (106535); Activation Of Nicotinic Acetylcholine Receptors Pathway (161027); Effects Of Botulinum Toxin Pathway (138028); ErbB2/ErbB3 Signaling Events Pathway (137955); Highly Calcium Permeable Nicotinic Acetylcholine Receptors Pathway (161029); Highly Calcium Permeable Postsynaptic Nicotinic Acetylcholine Receptors Pathway (161032); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534)

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

0.2 mg

425 USD