product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-amyloid beta [6E10]
catalog :
MBS488264
quantity :
0.2 mg
price :
425 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
[6E10]
application :
western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay
more info or order :
image
image 1 :
Immunohistochemical staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.
image 2 :
Western Blot using anti-Amyloid beta antibody 6E10 Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). frontal cortex and cerebellum lysate samples.
product information
catalog number :
MBS488264
products type :
Antibody
products full name :
Anti-amyloid beta [6E10]
products short name :
[amyloid beta]
products name syn :
[amyloid beta-peptide]
products gene name :
[A-beta]
clonality :
Monoclonal
isotype :
IgG, kappa
clone :
[6E10]
host :
Rabbit
reactivity :
Human
specificity :
This antibody binds to amino acid residues 1-16 of beta amyloid.
purity :
Purified. Protein A Affinity Purified
form :
PBS with 0.02% Proclin 300.
storage stability :
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
tested application :
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), EM
app notes :
This antibody recognizes human amyloid beta and can be used to detect amyloid in ELISAs, WB and tissue sections.
image1 heading :
Immunohistochemistry (IHC)
image2 heading :
Western Blot (WB)
other info1 :
Immunogen: Amyloid-beta peptide.
other info2 :
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. Publication Note: Describesthe use of this antibody in a sandwich ELISA to detect amyloid protein.
products categories :
Neurodegeneration; Alzheimers; Amyloid
products description :
Recombinant monoclonal antibody to amyloid beta. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6E10.
products references :
Pirttilä et al. J Neurol Sci. 1994 Dec 1;127(1):90-5. PMID:7699397
size1 :
0.2 mg
price1 :
425 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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