product summary
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company name :
MyBioSource
product type :
antibody
product name :
Anti-ED-B domain of fibronectin [A2]
catalog :
MBS488254
quantity :
0.2 mg
price :
425 USD
clonality :
monoclonal
host :
rabbit
conjugate :
nonconjugated
clone name :
[A2]
application :
western blot, ELISA, immunohistochemistry, enzyme immunoassay
more info or order :
image
image 1 :
Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody A2 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of A2 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticular staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.
product information
catalog number :
MBS488254
products type :
Antibody
products full name :
Anti-ED-B domain of fibronectin [A2]
products short name :
[ED-B domain of fibronectin]
products name syn :
[Extra domain-B containing fibronectin]
products gene name :
[EDB(+) FN]
clonality :
Monoclonal
isotype :
IgG, kappa
clone :
[A2]
host :
Rabbit
reactivity :
Human
specificity :
This antibody bind to human Extra-Domain containing fibronectin.
purity :
Protein A Affinity Purified
form :
PBS with 0.02% Proclin 300.
concentration :
1 mg/mL
storage stability :
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
tested application :
ELISA (EIA), Biacore, Western Blot (WB), Immunohistochemistry (IHC)-Fr.
app notes :
Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Extra domain-B containing fibronectin has been proposed marker of angiogenesis, has been shown to be expressed in a number of human cancers and in ocular neovascularization in patients with proliferative diabetic retinopathy.
image1 heading :
Immunofluorescence (IF)
other info1 :
Immunogen: 2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells. Antigen: ED-B domain of fibronectin. Antigen Description: 2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
other info2 :
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. Publication Note: Describes generation, using phage display, of antibodies with subnanomolar affinity against markers of angiogenesis.
products categories :
Phage display; Angiogenesis; Inflammation; Development; Cancer
products description :
Recombinant monoclonal antibody to ED-B domain of fibronectin. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the phage display antibodyA2.
products references :
Pini et al. J Biol Chem. 1998 Aug 21;273(34):21769-76. PMID:9705314
size1 :
0.2 mg
price1 :
425 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
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