catalog number :
MBS488092
products full name :
Anti-EGFR [7A7]
products short name :
[EGFR]
products name syn :
[ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor]
other names :
[epidermal growth factor receptor, partial]
products gene name :
[EGFR]
reactivity :
Human, Mouse
specificity :
In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
purity :
Protein A Affinity Purified
form :
Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
storage stability :
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
tested application :
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry - Paraffin (IHC-P), Immunohistochemistry - Frozen (IHC-F)
app notes :
Western Blot: 1:500-1:2,000. Immunofluorescence: 1:50-1:500. Immunohistochemistry (paraffin): 1:50-1:500. Immunohistochemistry (frozen): 1:50-1:500
other info1 :
Immunogen: Purified recombinant peptide produced in E. coli.
products categories :
Signal Transduction; Biotherapeuticresearch; Invivo; Cancer
products description :
Recombinant monoclonal antibody to EGFR. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 7A7. This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
products references :
Garrido G, Sanchez B, Rodriguez HM, Lorenzano P, Alonso D, Fernandez LE. 7A7 MAb: a new tool for the pre-clinical evaluation of EGFR-based therapies. Hybrid Hybridomics. 2004 Jun; 23(3):168-75. PMID:15312307