protein

GST T1-1, Recombinant Human

MBS483026

0.1 mg

410 USD

Recombinant Protein

GST T1-1, Recombinant Human

GST T1-1

Recombinant Human GST T1-1

glutathione transferase T1-1; Glutathione S-transferase theta-1; glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1; glutathione S-transferase theta 1; GST class-theta-1; Glutathione transferase T1-1

GST T1-1

GSTT1; GSTT1

GSTT1_HUMAN

Recombinant 6x His-tagged protein expressed in E Coli

240

Specific Activity: 8.8 Units/mg as assayed by the spectrophotometric determination of NADPH oxidation coupled to the glutathione peroxidase activity of GST T1-1 on cumene hydroperoxide (1.5 mM) in the presence of reduced glutathione (1 mM) in 100 mM NaPO4 (pH 7.0) at room temperature.

>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.

1.95 mg/mL total protein using the Bradford protein assay with BSA as a standard.

At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES

Storage Buffer: 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol. Procedural Notes: This purified product exhibits moderate enzymatic activity for Cumene hydroperoxide, the substrate that is most commonly used to assess the glutathione peroxidase activity of soluble GSTs. However, as is suspected for GST activity analysis using synthetic and natural substrates, low concentrations (high dilutions) of the enzyme may result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity towards GST substrates and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than Cumene hydroperoxide

Pemble S., et al., Biochem. J., 300 (Pt 1):271-276 (1994)

16923707

AAL31549.1

P30711

~25 kDa

Aflatoxin B1 Metabolism Pathway (198808); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343)

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. GSTT1 and GSTT2 share 55% amino acid sequence identity and both may play an important role in human carcinogenesis. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. This GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes, which are also located on chromosome 22, have been identified. [provided by RefSeq, Jun 2014]

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family. Protein type: Transferase; EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: cytosol. Molecular Function: glutathione transferase activity; glutathione peroxidase activity. Biological Process: glutathione metabolic process; xenobiotic metabolic process

0.1 mg

410 USD