protein

GST A3-3, Recombinant Rat

MBS480626

0.1 mg

450 USD

Recombinant Protein

GST A3-3, Recombinant Rat

[GST A3-3]

[Recombinant Rat GST A3-3]

[glutathione transferase A3-3; Glutathione S-transferase A3; glutathione S-transferase A3; GST class-alpha member 3; S-(hydroxyalkyl)glutathione lyase A3; glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3-3; glutathione S-transferase alpha 3; GST class-alpha member 3; Glutathione S-transferase A3-3]

[GST A3-3]

[GSTA3; GSTA3; GTA3; GSTA3-3]

GSTA3_HUMAN

Rat

> 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.

50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 5 mM EDTA and 50% glycerol.

2.07 mg/mL total protein using the Bradford protein assay with BSA as a standard.

Store at -20°C. Ships on dry ice. AVOID MULTIPLE FREEZE-THAW CYCLES.

Host Note: Rat recombinant protein expressed in E. coli. Specific Activity: 25.22 Units/mg using spectrophotometric determination of 1-chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

20977534

AAM33360.1

Q16772

23 kDa

Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (343); Metabolism Pathway (477135); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031)

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

GSTA3: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)- pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Belongs to the GST superfamily. Alpha family. Protein type: Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18; Transferase. Chromosomal Location of Human Ortholog: 6p12.1. Cellular Component: cytosol. Molecular Function: glutathione transferase activity. Biological Process: glutathione metabolic process; xenobiotic metabolic process

0.1 mg

450 USD