Anti-cPLA2, Mouse Polyclonal | MyBioSource MBS480031 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-cPLA2, Mouse Polyclonal

catalog :

MBS480031

quantity :

1 mg

price :

485 USD

clonality :

polyclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS480031

products type :

Antibody

products full name :

Anti-cPLA2, Mouse Polyclonal

products short name :

cPLA2

products name syn :

Anti-Mouse cPLA2

other names :

cytosolic phospholipase A2; Cytosolic phospholipase A2; cytosolic phospholipase A2; cPLA2; calcium-dependent phospholipid-binding protein; lysophospholipase; phosphatidylcholine 2-acylhydrolase; phospholipase A2 group IVA; phospholipase A2, group IVA (cytosolic, calcium-dependent); Phospholipase A2 group IVAIncluding the following 2 domains:Phospholipase A2 (EC:3.1.1.4)Alternative name(s):Phosphatidylcholine 2-acylhydrolase

products gene name :

cPLA2

other gene names :

PLA2G4A; PLA2G4A; PLA2G4; cPLA2-alpha; CPLA2; PLA2G4; cPLA2

uniprot entry name :

PA24A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Mouse

sequence length :

749

specificity :

Mouse

purity :

Ammonium Sulfate Fractionation

concentration :

1 mg/mL

storage stability :

At -70 degree C. AVOID REPEATED FREEZE THAW CYCLES. Has demonstrated stability at 4 degree C for greater than 1 month., Phosphate Buffered Saline; 0.05% Sodium Azide

tested application :

Western Blot (WB)

app notes :

Western Blot: 1-5 mug/mL

other info1 :

Storage Buffer: Phosphate Buffered Saline; 0.05% Sodium Azide. Procedural Notes: Yields a positive response with 10 mug of Raw cell line using alkaline phosphatase detection method.

products description :

Description: This polyclonal antibody is produced in rabbit and is provided as an IgG preparation specific for cytosolic Phospholipase A2 (cPLA2).

products references :

Channon, JY et al. JBC, 265: 5409-5413 (1990) Kramer, RM et al. JBC, 270: 14816-14823 (1995) Clark, JD et al. Cell, 65(6): 1043-1051 (1991)

ncbi gi num :

23943920

ncbi acc num :

NP_077734.1

ncbi gb acc num :

NM_024420.2

uniprot acc num :

P47712

ncbi mol weight :

85 kDa

ncbi pathways :

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); Acyl Chain Remodeling Of CL Pathway (645330); Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553)

ncbi summary :

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]

uniprot summary :

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A. Protein type: Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - linoleic acid; EC 3.1.1.4; EC 3.1.1.5; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; Phospholipase. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasmic membrane-bound vesicle; cytoplasm; mitochondrial inner membrane; cytosol. Molecular Function: phospholipase A2 activity; calcium-dependent phospholipid binding; calcium-dependent phospholipase A2 activity; calcium ion binding; lysophospholipase activity. Biological Process: icosanoid biosynthetic process; platelet activation; platelet activating factor biosynthetic process; phospholipid catabolic process; phospholipid metabolic process; icosanoid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation

size1 :

1 mg

price1 :

485 USD

more info or order :

MyBioSource product webpage