Anti-CCNE1 Mouse mAb | MyBioSource MBS476572 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CCNE1 Mouse mAb

catalog :

MBS476572

quantity :

0.1 mL

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[5F8B4]

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis using CCNE1 mAb against human CCNE1 (AA

image 2 :

Western blot analysis using CCNE1 mAb against HEK293 (1) and CCNE1 (AA

image 3 :

Flow cytometric analysis of K562 cells using CCNE1 mouse mAb (green) and negative control (red).

product information

catalog number :

MBS476572

products type :

Antibody

products full name :

Anti-CCNE1 Mouse mAb

products short name :

[CCNE1]

products name syn :

[CCNE]

other names :

[CCNE1; G1/S-specific cyclin-E1; G1/S-specific cyclin-E1; cyclin E1]

products gene name :

[CCNE1]

products gene name syn :

[CCNE]

other gene names :

[CCNE1; CCNE1; CCNE; pCCNE1; CCNE]

clonality :

Monoclonal

isotype :

IgG1

clone :

[5F8B4]

host :

Mouse

reactivity :

Human

sequence length :

434

form :

Purified antibody in PBS with 0.05% sodium azide.

storage stability :

Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CCNE1 mouse mAb with DAB staining.

other info1 :

Immunogen: Purified recombinant fragment of human CCNE1 (AA: 307-410) expressed in E Coli. Antibody Diluents: 5% w/v nonfat dry milk, 1 x PBS, 0.1%Tween-20

products description :

Entrez Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

ncbi gi num :

958303630

ncbi acc num :

JAO50970.1

ncbi mol weight :

ncbi pathways :

ATM Signaling Pathway (920969); Androgen Receptor Signaling Pathway (198806); Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway (1268699); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (198811); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle, Mitotic Pathway (1269763); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016]

uniprot summary :

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described. Protein type: Activator; Cell cycle regulation; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 19q12. Cellular Component: cytosol; nucleoplasm; nucleus. Molecular Function: protein binding. Biological Process: G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; protein amino acid phosphorylation

size1 :

0.1 mL

price1 :

335 USD

more info or order :

MyBioSource product webpage