Anti-ITK Mouse mAb | MyBioSource MBS476195 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-ITK Mouse mAb

catalog :

MBS476195

quantity :

0.1 mL

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[7G6-3D6-9D7]

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis using ITK mAb against human ITK (AA:2-120) recombinat protein. (Expected MW is 39.7 kDa)

image 2 :

Western blot analysis using ITK mouse mAb against Jurkat cell lysate.

image 3 :

Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

product information

catalog number :

MBS476195

products type :

Antibody

products full name :

Anti-ITK Mouse mAb

products short name :

[ITK]

products name syn :

[EMT; LYK; PSCTK2; MGC126257; MGC126258]

other names :

[ITK; Tyrosine-protein kinase ITK/TSK; tyrosine-protein kinase ITK/TSK; IL2 inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk]

products gene name :

[ITK]

products gene name syn :

[EMT; LYK; PSCTK2; MGC126257; MGC126258]

other gene names :

[ITK; ITK; EMT; LYK; LPFS1; PSCTK2; EMT; LYK; IL-2-inducible T-cell kinase]

clonality :

Monoclonal

isotype :

IgG1

clone :

[7G6-3D6-9D7]

host :

Mouse

reactivity :

Human

sequence length :

620

form :

Ascitic fluid containing 0.03% sodium azide

storage stability :

Store at 4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunocytochemistry (ICC)

app notes :

Western Blot (WB) 1/500-1/2000. Immunocytochemistry (ICC) 1/200-1/1000. Flow Cytometry (FCM) 1/200-1/400. ELISA (EIA) 1/10000.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).

other info1 :

Immunogen: Purified recombinant fragment of human ITK expressed in E Coli. Conjugation: Unconjugated. Antibody Type: Primary antibody. Subcellular Location: Cytoplasm, Nucleus. Modification: Unmodification

products description :

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

ncbi gi num :

399658

ncbi acc num :

BAA02873.1

ncbi pathways :

Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class I PI3K Signaling Events Pathway (138022); FCERI Mediated Ca+2 Mobilization Pathway (833827); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825); Fc-epsilon Receptor I Signaling In Mast Cells Pathway (138050); Generation Of Second Messenger Molecules Pathway (106417); Immune System Pathway (106386)

ncbi summary :

uniprot summary :

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. Plays an essential role in regulation of the adaptive immune response. Contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Is recruited to the cell membrane following activation of the T-cell receptor (TCR). It is phosphorylated by LCK, followed by autophosphorylation leading to its full activation. Phosphorylates PLCG1. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization. Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family. Chromosomal Location of Human Ortholog: 5q33.3. Cellular Component: cytosol; extrinsic to internal side of plasma membrane. Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding. Biological Process: adaptive immune response; cellular defense response; cytokine production; innate immune response; NK T cell differentiation; phospholipase C activation; regulation of cell proliferation; signal transduction; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Lymphoproliferative Syndrome 1

size1 :

0.1 mL

price1 :

335 USD

more info or order :

MyBioSource product webpage