Anti-SIRT6 Mouse mAb | MyBioSource MBS475406 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-SIRT6 Mouse mAb

catalog :

MBS475406

quantity :

0.1 mL

price :

335 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[6C9-D10-D3]

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from Jurkat, MCF7, HCT116, K562, COS7, C2C12, 3T3 and Hela cell lysates using SIRT6 mouse mAb (1:500 diluted).Predicted band size:42, 36KDa.Observed band size:42, 36KDa.

image 2 :

Immunofluorescent analysis of Hela cells fixed fixed by 4% paraformaldehyde and using SIRT6 mouse mAb (dilution 1:100).

image 3 :

Immunoprecipitation analysis of Hela cell lysates using SIRT6 mouse mAb.

product information

catalog number :

MBS475406

products type :

Antibody

products full name :

Anti-SIRT6 Mouse mAb

products short name :

[SIRT6]

products name syn :

[2810449N18Rik; AI043036; Mono ADP ribosyltransferase sirtuin 6; NAD-dependent protein deacetylase sirtuin-6; Regulatory protein SIR2 homolog 6; Regulatory protein SIR2 homolog; SIR2 like 6; SIR2 like protein 6; Sir2 related protein type 6; SIR2-like protein 6; SIR2]

other names :

[NAD-dependent protein deacetylase sirtuin-6 isoform 2; NAD-dependent protein deacetylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; sirtuin 6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6]

products gene name :

[SIRT6]

products gene name syn :

[SIR2L6.]

other gene names :

[SIRT6; SIRT6; SIR2L6; SIR2L6]

clonality :

Monoclonal

isotype :

IgG1

clone :

[6C9-D10-D3]

host :

Mouse

reactivity :

Human, Mouse, Rat, Monkey

sequence length :

328

purity :

Affinity Purified

form :

PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

storage stability :

Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

app notes :

WB: 1:500. ICC: 1:100

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Immunoprecipitation (IP)

other info1 :

Immunogen: Purified recombinant human SIRT6 protein expressed in E Coli. Sensitivity: This antibody detects endogenous levels of SIRT6 and does not cross-react with related proteins. Sequence Similarities: Belongs to the sirtuin family. Class IV subfamily.Contains 1 deacetylase sirtuin-type domain.

other info2 :

Subcellular Location: Nucleus. Antibody Diluents: 5% w/v nonfat dry milk, 1 x PBS, 0.1%Tween-20

products categories :

Epigenetics and Nuclear Signaling; Nuclear Signaling Pathways; NFkB pathway; Epigenetics and Nuclear Signaling; Chromatin Modifying Enzymes; Acetylation; HDACs; Class III / Sir2 class

products description :

Entrez Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been det

ncbi gi num :

300797597

ncbi acc num :

NP_001180214.1

ncbi gb acc num :

NM_016539

uniprot acc num :

Q8N6T7

ncbi mol weight :

ncbi pathways :

Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); SREBF And MiR33 In Cholesterol And Lipid Homeostasis Pathway (755438); Signaling Events Mediated By HDAC Class I Pathway (137997)

ncbi summary :

This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

uniprot summary :

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: nuclear telomeric heterochromatin; nucleolus; nucleoplasm; nucleus. Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; zinc ion binding. Biological Process: positive regulation of chromatin silencing at telomere; positive regulation of telomere maintenance; protein amino acid ADP-ribosylation

size1 :

0.1 mL

price1 :

335 USD

more info or order :

MyBioSource product webpage