protein

PAK6 (N-terminal region) Blocking Peptide

MBS474017

0.05 mg

130 USD

Peptide

PAK6 (N-terminal region) Blocking Peptide

PAK6 (N-terminal) Peptide

PAK6 (N-terminal) Peptide

serine/threonine-protein kinase PAK 6; Serine/threonine-protein kinase PAK 6; serine/threonine-protein kinase PAK 6; PAK-5; PAK-6; OTTHUMP00000160372; p21-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; PAK-5; p21-activated kinase 6

PAK6; PAK6; PAK5; PAK5

PAK6_HUMAN

681

Peptide Sequence: A synthetic peptide corresponding to amino acids in the N-terminal region of human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.

The peptide is specifically recognized by anti-PAK6 (N-terminal region) antibody (PP1511) in ELISA, and has been shown to block the reactivity of PP1511 during Western blot. In addition, the peptide is recommended for use in blocking PP1511 reactivity in immunocytochemistry.

Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.

Store at -20 degree C. Stable for 1 year.

Antibody Blocking: 1 ug/ml. ELISA: 10-100 ng/well

p21-activated kinase 6 (PAK6) is a member of the PAK family of serine/threonine kinases. These kinases have a highly conserved amino-terminal Cdc42/Rac interactive binding domain and a carboxylterminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. PAK6 interacts with the androgen receptor, a steroid hormone transcription factor involved in male sexual differentiation and development. PAK6 is highly expressed in testis and prostate tissues. Regulation of PAK6 kinase activity occurs through multiple sites of phosphorylation. Activation of PAK6 requires autophosphorylation of Ser-560 and MKK-6 induced phosphorylation of Tyr-566. In addition, p38 MAPK can phosphorylate Ser-165, which increases PAK6 kinase activity. Thus, multiple signaling pathways may regulate the activity of PAK6 through differential phosphorylation.

Kaur, R. et al. (2005) J. Biol. Chem. 280(5) :3323. Schrantz, N. et al. (2004) J. Biol. Chem. 279(3):1922. Lee, S.R. et al. (2002) Mol. Endocrin. 16(1):85.

191252782

NP_001122101.1

NM_001128629.1

Q9NQU5

74738 Da

Activation Of Rac Pathway 119530!!Androgen Receptor Signaling Pathway 198806!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!ErbB Signaling Pathway 83049!!ErbB Signaling Pathway 458!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Function: The activated kinase acts on a variety of targets. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Subunit structure: Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. By similarity. Interacts with the androgen receptor. Post-translational modification: Autophosphorylated when activated by CDC42/p21. By similarity. Ref.4 Ref.5 Ref.6. Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain.

0.05 mg

130 USD