antibody

WAVE2 (Central region)

MBS472070

0.1 ml

280 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA

Antibody

WAVE2 (Central region)

WAVE2 (Central region)

Wiskott-Aldrich syndrome verproline-homologous protein 2; Scar2; WASF2

wiskott-Aldrich syndrome protein family member 2 isoform 2; WASF2 protein; wiskott-Aldrich syndrome protein family member 2; IMD2; protein WAVE-2; OTTHUMP00000003471; WASP family protein member 2; WASP family Verprolin-homologous protein 2; verprolin homology domain-containing protein 2; suppressor of cyclic-AMP receptor (WASP-family); WAS protein family, member 2

WASF2; WASF2; SCAR2; WAVE2; dJ393P12.2

Q05BU7_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody was affinity purified using WAVE2 (Central region) peptide (without carrier). The antibody detects a 78 kDa* protein corresponding to the molecular mass of WAVE2 on SDS-PAGE immunoblots of rat PC12 cells.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000

Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the central region of human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has low homology to similar regions in WAVE1 and WAVE3.

The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an Nterminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6. Leng, Y. et al. (2005) PNAS 102(4):1098. Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296. Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.

319655728

NP_001188333.1

NM_001201404.1

Q05BU7

21,410 Da

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!E-cadherin Signaling In The Nascent Adherens Junction Pathway 138005!!ErbB1 Downstream Signaling Pathway 138057!!Fc Gamma R-mediated Phagocytosis Pathway 114228!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!RAC1 Signaling Pathway 169355!!Regulation Of Actin Cytoskeleton Pathway 198874

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

0.1 ml

280 USD