product summary
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company name :
MyBioSource
product type :
antibody
product name :
RhoA (Ser-188), phospho-specific
catalog :
MBS472056
quantity :
0.1 ml
price :
380 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS472056
products type :
Antibody
products full name :
RhoA (Ser-188), phospho-specific
products short name :
RhoA (Ser-188)
products name syn :
Gb; p21
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human, Rat, Mouse
specificity :
This antibody was affinity purified using phospho-Rho (Ser-188) peptide (without carrier). The antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein.
purity :
Affinity Purified
form :
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
storage stability :
Store at -20 degree C. Do not aliquot. Stable for 1 year.
tested application :
ELISA, Western Blot
app notes :
ELISA: 1:2000. Western Blot: 1:1000
other info1 :
Immunogen: Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. This peptide sequence is highly conserved in rat and mouse RhoA, but is not found in RhoB or RhoC proteins.
products description :
Rho (A, B, & C) proteins are members of the Ras superfamily of GTPases. These proteins regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and an inactive GDPbound form. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The Rho proteins RhoA, RhoB, and RhoC are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser-188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser-188 is an important site for negative regulation of RhoA activity.
products references :
Lang, P. et al. (1996) EMBO J. 15:510. Dong, J.M. et al. (1998) J. Biol. Chem. 273(35):22554. Sawada, N. et al. (2001) Biochem. Biophys. Res. Commun. 280:798. Ellerbroek, S.M. et al. (2003) J. Biol. Chem. 278(21):19023.
size :
0.1 ml
price :
380 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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