antibody

Anti-Plexin-A1 (Sema Domain)

MBS472054

0.1 ml

380 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, immunoprecipitation

Antibody

Anti-Plexin-A1 (Sema Domain)

Plexin A1 (Sema Domain)

Plexin A1 (Sema Domain); PLXN1; NOV

plexin A1; cDNA FLJ44113 fis, clone TESTI4046487, highly similar to Mus musculus plexin A1 (Plxna1); plexin-A1; plexin 1; OTTHUMP00000215966; semaphorin receptor NOV; plexin A1

PLXNA1; NOV; NOVP; PLXN1; PLEXIN-A1; DKFZp761P19121

Q6ZTY7_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

1873

The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain lysates and Cos-7 cells transfected with mouse Plexin-A1.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation

ELISA: 1:2000. Immunocytochemistry: 1:100. Immunoprecipitation: 5-10ml. Western Blot: 1:1000

Immunogen: Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the semaphorin domain in human Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429. Tamagnone, L. et al. (1999) Cell 99:71.

49355818

NP_115618.2

NM_032242.2

Q6ZTY7

208419 Da

Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 366334!!Axon Guidance Pathway 105688!!CRMPs In Sema3A Signaling Pathway 119525!!Other Semaphorin Interactions Pathway 119526!!SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway 119523!!Sema3A PAK Dependent Axon Repulsion Pathway 119524!!Sema3A PAK Dependent Axon Repulsion Pathway 366340!!Semaphorin Interactions Pathway 119519

0.1 ml

380 USD