antibody

Anti-PLC-gamma1 (Tyr-775), phospho-specific

MBS472053

0.1 mL

380 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, immunoprecipitation

Antibody

Anti-PLC-gamma1 (Tyr-775), phospho-specific

PLCg1 (Tyr-775)

PLCg1 (Tyr-775); phospho-specific; Phospholipase C gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 isoform a; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; PLC-gamma-1; OTTHUMP00000031787; OTTHUMP00000178982; phospholipase C-II; phospholipase C-148; phosphoinositidase C; phospholipase C-gamma-1; inositoltrisphosphohydrolase; phosphoinositide phospholipase C; phosphatidylinositol phospholipase C; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-gamma-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; phospholipase C, gamma 1 (formerly subtype 148); 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; phospholipase C, gamma 1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; PLC-II; Phospholipase C-gamma-1

PLCG1; PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; PLC1

PLCG1_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

1291

This antibody was cross-adsorbed to a non-specific phospho-tyrosine peptide then affinity-purified using phospho-PLC-gamma-1 (Tyr-775) peptide. The antibody detects a 150 kDa* protein in human Jurkat and A431 cells treated with pervanadate, but is not observed in untreated cells.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation

ELISA: 1:2000. Immunocytochemistry: 1:100. Immunoprecipitation: 1:100. Western Blot: 1:1000

Immunogen: PLC-gamma-1 (Tyr-775) synthetic peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 775 in human PLC-gamma-1. This sequence has high homology to the conserved site in rat and mouse PLC-gamma-1, and has low homology to PLC-gamma-2.

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLC-beta, PLC-gamma, PLC-delta, and PLC-epsilon. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLC-delta is activated by both receptor and nonreceptor tyrosine kinases. PLC-gamma-1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLC-gamma-1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity.

Obermeier, A. et al. (1996) EMBO J. 15:73. Chen, P. et al. (1996) J Cell. Biol. 134:689. Serrano, C.J. et al. (2005) J. Immunol. 174:6233.

33598948

NP_002651.2

NM_002660.2

P19174

148530 Da

Adaptive Immunity Signaling Pathway (366160); Axon Guidance Pathway (105688); B Cell Receptor Signaling Pathway (198909); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Cell Surface Interactions At The Vascular Wall Pathway (106062); Class I PI3K Signaling Events Pathway (138022); Down-stream Signal Transduction Pathway (106385); Downstream Signaling Of Activated FGFR Pathway (160957); E-cadherin Signaling In Keratinocytes Pathway (138002)

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Function: Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Ref.17. Catalytic activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. Cofactor: Calcium. Subunit structure: Interacts with AGAP2 via its SH3 domain. By similarity. Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK. By similarity. Interacts with RALGPS1. Interacts (via SH3 domain) with HEV ORF3 protein. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.17. Subcellular location: Cell projection lamellipodium. Cell projection ruffle. Note: Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. Ref.17. Domain: The SH3 domain mediates interaction with CLNK. By similarity. The SH3 domain also mediates interaction with RALGPS1. Ref.26. Post-translational modification: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ. Ref.11 Ref.12 Ref.14 Ref.15 Ref.16 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24Ubiquitinated by CBLB in activated T-cells. Sequence similarities: Contains 1 C2 domain.Contains 1 EF-hand domain.Contains 2 PH domains.Contains 1 PI-PLC X-box domain.Contains 1 PI-PLC Y-box domain.Contains 2 SH2 domains.Contains 1 SH3 domain.

0.1 mL

380 USD