antibody

PKC-alpha (Ser-657/Tyr-658), phospho-specific

MBS472052

0.1 ml

380 USD

polyclonal

rabbit

nonconjugated

human, rat

western blot, ELISA, immunoprecipitation

Antibody

PKC-alpha (Ser-657/Tyr-658), phospho-specific

PKC a (Ser-657/Tyr-658)

PKC a (Ser-657/Tyr-658); phospho-specific; Protein Kinase C alpha

Protein kinase C alpha type; Protein kinase C alpha type; protein kinase C alpha type; PKC-A; aging-associated gene 6; protein kinase C, alpha

PRKCA; PRKCA; AAG6; PKCA; PRKACA; MGC129900; MGC129901; PKC-alpha; PKCA; PRKACA

KPCA_HUMAN

Polyclonal

Rabbit

Human, Rat, Rabbit

672

This antibody detects an 82kDa* protein corresponding to the molecular mass of phosphorylated PKCa on SDSPAGE immunoblots of neonatal rat brain lysate. Similar results were observed in human SKN-SH, endothelial, and HeLa cells, as well as rabbit spleen fibroblasts and rat pituitary cells. In immunoprecipitation experiments with various PKC isoforms, this antibody detected PKCa and PKCb but not other PKC isoforms in rat brain lysate.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot, Immunoprecipitation

ELISA: 1:2000. Immunoprecipitation: 5-10ml. Western Blot: 1:1000

Immunogen: Phospho-PKCa (Ser-657/Tyr-658) synthetic peptide corresponds to amino acid residues around serine 657 and tyrosine 658 of human PKCa. This sequence is similar to the conserved sites in rat and mouse, and has high homology to dual sites in human, rat, and mouse PKCbI (Ser-661/Tyr-662) and PKCg (Thr-674/Tyr675).

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta-I, beta-II, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, Fc-epsilon-RI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC-beta-I and PKC-alpha, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

Kawakami et al. (2003) Proc. Natl. Acad. Sci. USA 100:9470-9475. Nishizuka, Y. (1988) Nature 334:661.

125549

P17252.3

P17252

P17252

76,750 Da

ATF-2 Transcription Factor Network Pathway 138006!!African Trypanosomiasis Pathway 194384!!African Trypanosomiasis Pathway 194323!!Aldosterone-regulated Sodium Reabsorption Pathway 130626!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Ca-dependent Events Pathway 106497!!CaM Pathway 106468

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq]

Function: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. Ref.9PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Ref.9. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain. Subunit structure: Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1. By similarity. Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. Interacts with PICK1 (via PDZ domain). Interacts with TRIM41. Ref.7 Ref.8 Ref.9 Ref.13. Subcellular location: Cytoplasm. By similarity. Cell membrane; Peripheral membrane protein. By similarity. Nucleus. Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 C2 domain.Contains 2 phorbol-ester/DAG-type zinc fingers.Contains 1 protein kinase domain.

0.1 ml

380 USD