catalog number :
MBS472051
products full name :
Anti-Phosphotyrosine
products short name :
Phosphotyrosine
products name syn :
Phosphotyrosine; Ptyr
reactivity :
Human, Rat, Mouse, Chicken
specificity :
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
purity :
Affinity Purified
form :
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
storage stability :
Store at -20 degree C. Do not aliquot. Stable for 1 year.
tested application :
ELISA, Western Blot
app notes :
ELISA: 1:2000. Immunoprecipitation: 1:100. Western Blot: 1:1000
other info1 :
Immunogen: Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
products description :
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications.Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
products references :
Ross, A.H. et al. (1981) Nature 294:654. Wang, J.Y.J. (1988) Anal. Biochem 172:1.