antibody

Anti-Paxillin (Ser-178), phospho-specific

MBS472047

0.1 mL

380 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA

Antibody

Anti-Paxillin (Ser-178), phospho-specific

Paxillin (Ser-178)

Paxillin (Ser-178); phospho-specific; Paxillin

paxillin; Paxillin; paxillin; myocardial ischemic preconditioning associated protein 7; paxillin

Pxn; Pxn; Pxn

PAXI_RAT

Polyclonal

Rabbit

Human, Rat, Mouse

586

This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:500

Immunogen: Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.

Huang, C. et al. (2003) Nature 424:219-223. Huang, C. et al. (2004) Cell Cycle 3(1):4-6. Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.

58865866

NP_001012147.1

NM_001012147.1

Q66H76

63888 Da

Androgen Receptor Signaling Pathway (198529); Bacterial Invasion Of Epithelial Cells Pathway (149774); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); EGFR1 Signaling Pathway (198427); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478); Gab1 Signalosome Pathway (367990); IL-3 Signaling Pathway (198504)

Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Subunit structure: Binds to vinculin, to the SH3 domain of c-SRC and to focal adhesion kinase. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10. By similarity. Interacts with PARVA and TGFB1I1. Ref.2 Ref.3. Subcellular location: Cytoplasm cytoskeleton. By similarity. Cell junction focal adhesion. Post-translational modification: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. By similarity. Ref.4. Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.

0.1 mL

380 USD