Anti-N-WASP (Tyr-256), phospho-specific | MyBioSource MBS472041 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-N-WASP (Tyr-256), phospho-specific

catalog :

MBS472041

quantity :

0.1 mL

price :

380 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS472041

products type :

Antibody

products full name :

Anti-N-WASP (Tyr-256), phospho-specific

products short name :

N-WASP (Tyr-256)

products name syn :

N-WASP (Tyr-256); phospho-specific; Neural Wiskott-Aldrich syndrome protein; WASL

other names :

neural Wiskott-Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like; Wiskott-Aldrich syndrome-like

other gene names :

WASL; WASL; NWASP; N-WASP; MGC48327; DKFZp779G0847

uniprot entry name :

WASL_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, Rat, Mouse

specificity :

This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier). The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine.

purity :

Affinity Purified

form :

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

storage stability :

Store at -20 degree C. Do not aliquot. Stable for 1 year.

tested application :

ELISA, Western Blot

app notes :

ELISA: 1:20000. Western Blot: 1:10000

other info1 :

Immunogen: Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.

products description :

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.

products references :

Baba, Y. et al. (1999) Blood 93:2003. Torres, E. & Rosen, M.K. (2003) Mol Cell 11:1215. Wu, X. et al. (2004) J Biol Chem 279(10):9565.

ncbi gi num :

51702526

ncbi acc num :

NP_003932.3

ncbi gb acc num :

NM_003941.2

uniprot acc num :

O00401

ncbi mol weight :

54,827 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); CDC42 Signaling Events Pathway (137994); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); DCC Mediated Attractive Signaling Pathway (161000); EGF Receptor (ErbB1) Signaling Pathway (137970)

ncbi summary :

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]

uniprot summary :

Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.11. Subunit structure: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. By similarity. Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli EspF-like protein encoded on prophage U. Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF-like protein encoded on prophage U. Ref.4 Ref.5 Ref.6 Ref.11. Subcellular location: Cytoplasm cytoskeleton. By similarity. Nucleus. By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Sequence similarities: Contains 1 CRIB domain.Contains 1 WH1 domain.Contains 2 WH2 domains.

size1 :

0.1 mL

price1 :

380 USD

more info or order :

MyBioSource product webpage