antibody

Anti-N-WASP

MBS472039

0.1 ml

380 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA

Antibody

Anti-N-WASP

N-WASP

N-WASP; Neural Wiskott-Aldrich syndrome protein; WASL

neural Wiskott-Aldrich syndrome protein; Neural Wiskott-Aldrich syndrome protein; neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like; Wiskott-Aldrich syndrome-like

WASL; WASL; NWASP; N-WASP; MGC48327; DKFZp779G0847

WASL_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa* proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa* WASP protein in Jurkat cell lysate.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000

Immunogen: N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP. This N-WASP peptide sequence is 100% to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP sequence.

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.

Cory, G.O. et al. (2003) Mol Cell. 11(5):1229-39. Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.

51702526

NP_003932.3

NM_003941.2

O00401

54,827 Da

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Axon Guidance Pathway 105688!!Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!CDC42 Signaling Events Pathway 137994!!Chemokine Signaling Pathway 99051!!Chemokine Signaling Pathway 96864!!DCC Mediated Attractive Signaling Pathway 161000!!EGF Receptor (ErbB1) Signaling Pathway 137970

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq]

Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Ref.11. Subunit structure: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. By similarity. Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli EspF-like protein encoded on prophage U. Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF-like protein encoded on prophage U. Ref.4 Ref.5 Ref.6 Ref.11. Subcellular location: Cytoplasm cytoskeleton. By similarity. Nucleus. By similarity. Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Sequence similarities: Contains 1 CRIB domain.Contains 1 WH1 domain.Contains 2 WH2 domains.

0.1 ml

380 USD