antibody

N-Cadherin (Tyr-820), phospho-specific

MBS472036

0.1 mL

380 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA

Antibody

N-Cadherin (Tyr-820), phospho-specific

N-Cadherin (Tyr-820)

Cadherin-2; Neural-Cadherin; CD325 antigen

N-cadherin; Cadherin-2; cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; OTTHUMP00000066304; OTTHUMP00000067378; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; cadherin 2, type 1, N-cadherin (neuronal); CDw325; Neural cadherin

CDH2; CDH2; CDHN; NCAD; CD325; CDw325; CDHN; NCAD

CADH2_HUMAN

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody was cross-adsorbed to phospho-NCadherin (Tyr-860) and unphosphorylated N-cadherin (Tyr-820) peptides before affinity purification using phospho-N-cadherin (Tyr-820) peptide. The purified antibody detects a 130 kDa* band corresponding to Ncadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but is not detected in untreated cells.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000

Immunogen: Phospho-N-Cadherin (Tyr-820) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved site in Rcadherin.

Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr -886. Phosphorylation of Tyr-860 can disrupt cadherin binding to beta-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.

Xu, Y. et al. (1997) J. Biol. Chem. 272(21):13463. Xu, Y. & Carpenter, G. (1999) J. Cell. Bioch. 75:264. Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.

253483

P19022

99,809 Da

Adherens Junctions Interactions Pathway (119533); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); CDO In Myogenesis Pathway (160998); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-cell Junction Organization Pathway (119532); FGF Signaling Pathway (137989); Myogenesis Pathway (160997)

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Subunit structure: Interacts with CDCP1. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway. By similarity. Ref.10. Subcellular location: Cell membrane; Single-pass type I membrane protein. Sequence similarities: Contains 5 cadherin domains.

0.1 mL

380 USD