antibody

Inducible Nitric Oxide Synthase

MBS472029

0.1 mL

280 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA

Antibody

Inducible Nitric Oxide Synthase

Inducible Nitric Oxide Synthase

iNOS; NOS type II; Inducible nitric oxide synthase; Hepatocyte NOS

inducible nitric oxide synthase; Nitric oxide synthase, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2

iNOS; Nos2

NOS2_RAT

Polyclonal

Rabbit

Human, Rat, Mouse

1147

The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages treated with IFNg and LPS.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:4000. Western Blot: 1:2000

Immunogen: Amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.

Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343. Xie, Q.W. et al. (1992) Science 256:225.

2935553

AAC83553.1

AF049656.1

Q06518

130,628 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Catalytic activity: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+. Cofactor: Heme group.Binds 1 FAD.Binds 1 FMN.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. Enzyme regulation: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Subunit structure: Homodimer. Binds SLC9A3R1. Tissue specificity: In normal kidney, expressed primarily in the medullary thick ascending limb, with minor amounts in the medullary collecting duct and vasa recta bundle. Induction: By interferon gamma and lipopolysaccharides (LPS). Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain. Caution: sequence Was originally (Ref.9) thought to originate from human but appears to be from rat.

0.1 mL

280 USD