product summary
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company name :
MyBioSource
product type :
antibody
product name :
Blasticidin S Deaminase
catalog :
MBS472010
quantity :
0.1 mL
price :
380 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot, ELISA
more info or order :
product information
catalog number :
MBS472010
products type :
Antibody
products full name :
Blasticidin S Deaminase
products short name :
Blasticidin S Deaminase
products name syn :
BSD
other names :
Blasticidin-S deaminase; Blasticidin-S deaminase
other gene names :
bsd; bsd
uniprot entry name :
BSD_ASPTE
clonality :
Polyclonal
host :
Rabbit
sequence length :
130
specificity :
This antibody was affinity purified using the N-terminal Bsd peptide (without carrier). The antibody detects a 13 kDa* protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.
purity :
Affinity Purified
form :
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
storage stability :
Store at -20 degree C. Do not aliquot. Stable for 1 year.
tested application :
ELISA, Western Blot
app notes :
ELISA: 1:2000. Western Blot: 1:1000. ICC: 1:100
other info1 :
Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
products description :
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. Catalog# BP1231 is a Bsd antibody that can detect Bsd expression in cells transfected with blasticidin-selectable constructs. This antibody is useful for detection of successful gene transfer in constructs that utilize blasticidin selection.
products references :
Kimura M, et al. (1994). Biochim Biophys Acta. 1219(3):653-9. Izumi M, et al. (1991). Exp Cell Res. 197(2):229-33.
ncbi gi num :
143955286
ncbi acc num :
P0C2P0.1
ncbi gb acc num :
P0C2P0
uniprot acc num :
P0C2P0
ncbi mol weight :
13,468 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S. Catalytic activity: Blasticidin S + H2O = deaminohydroxyblasticidin S + NH3. Cofactor: Zinc. Subunit structure: Homotetramer. Ref.4. Sequence similarities: Belongs to the cytidine and deoxycytidylate deaminase family.
size1 :
0.1 mL
price1 :
380 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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