antibody

Anti-beta-Catenin (Tyr-86), phospho-specific

MBS472005

0.1 mL

380 USD

polyclonal

rabbit

nonconjugated

phosphorylated

human, mouse, rat, zebrafish

western blot, ELISA

Antibody

Anti-beta-Catenin (Tyr-86), phospho-specific

b-Catenin (Tyr-86)

b-Catenin (Tyr-86); phospho-specific; Catenin beta

b-catenin; Catenin (Cadherin-associated protein), beta 1; catenin beta-1; beta-catenin; catenin (cadherin-associated protein), beta 1

ctnnb1; ctnnb1; ctnnb; id:ibd2058; wu:fb73e10; wu:fi81c06; wu:fk25h01

Q7ZU14_DANRE

Polyclonal

Rabbit

Human, Rat, Mouse

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho- beta -Catenin (Tyr-86) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta -Catenin on SDS-PAGE immunoblots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells.

Affinity Purified

Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Store at -20 degree C. Do not aliquot. Stable for 1 year.

ELISA, Western Blot

ELISA: 1:2000. Western Blot: 1:1000. ICC: 1:100

Immunogen: Phospho-beta-Catenin (Tyr-86) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 86 of human beta-Catenin. This peptide sequence is highly conserved in rat and mouse beta-Catenin.

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of ECadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 654 in the C terminal armadillo repeat of beta-Catenin. This phosphorylation regulates beta-Catenin binding to E cadherin. Thus, sitespecific tyrosine phosphorylation of beta-Catenin may regulate specific protein-protein interactions leading to changes in cell adhesion.

Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246. Roura, S. et al. (1999) J Biol Chem. 274(51) :36734. Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287-2297. Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225-2230.

1125100

Q7ZU14

85,520 Da

Adherens Junction Pathway (119268); Adherens Junction Pathway (481); Adherens Junctions Interactions Pathway (365024); Androgen Receptor Signaling Pathway (198968); Apoptosis Pathway (364682); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (364704); Apoptotic Cleavage Of Cellular Proteins Pathway (364702); Apoptotic Execution Phase Pathway (364701); B Cell Receptor Signaling Pathway (198947); Beta-catenin Phosphorylation Cascade Pathway (364739)

0.1 mL

380 USD