product summary
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company name :
MyBioSource
product type :
antibody
product name :
PKC (alpha, beta, gamma)
catalog :
MBS470024
quantity :
0.1 ml
price :
280 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
product information
catalog number :
MBS470024
products type :
Antibody
products full name :
PKC (alpha, beta, gamma)
products short name :
PKC (alpha, beta, gamma)
products name syn :
Protein Kinase C alpha
clonality :
Monoclonal
isotype :
IgG1
host :
Mouse
reactivity :
Human, Rat, Mouse
specificity :
This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKCa, PKCb, and PKCg on SDS-PAGE immunoblots of neonatal rat brain lysates. Similar results were observed in human and mouse lysates. Immunoprecipitation experiments with various PKC isoforms demonstrated this antibody detects PKCa, PKCb, and PKCg, but not other PKC isoforms expressed in rat brain lysate.
purity :
Protein A Purified
form :
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ml phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
storage stability :
Store at -20 degree C. Do not aliquot. Stable for 1 year.
tested application :
ELISA, Western Blot, Immunocytochemistry, Immunoprecipitation
app notes :
ELISA: 1:2000. Immunocytochemistry: 1:100. Immunoprecipitation: 1-5ml. Western Blot: 1:1000
other info1 :
Immunogen: Clone (M110) was generated from a recombinant human PKCg that included amino acids residues 499-697.
products description :
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (alpha, beta-I, beta-II, and gamma) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, Fc-epsilon-RI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKC-beta-I and PKC-alpha, respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.
products references :
Kawakami et al. (2003) Proc. Natl. Acad. Sci. USA 100:9470-9475. Nishizuka, Y. (1988) Nature 334:661.
size :
0.1 ml
price :
280 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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